About Elisa

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies and hormones. In an ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measureable product. The most crucial element of the detection strategy is a highly specific antibody-antigen interaction.
ELISAs are typically performed in 96-well (or 384-well) polystyrene plates, which will passively bind antibodies and proteins. It is this binding and immobilization of reagents that makes ELISAs so easy to design and perform. Having the reactants of the ELISA immobilized to the microplate surface makes it easy to separate bound from non-bound material during the assay. This ability to wash away nonspecifically bound materials makes the ELISA a powerful tool for measuring specific analytes within a crude preparation.

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ELISA Technical Resources Center

ELISA Principle

ELISA Principle

Western blotting, ELISAs, and IHC are all immunoassays, and so all share in common the same basic…

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ELISA Sample Preparation

ELISA Sample Preparation

Every immunoassay protocol begins with sample preparation. Western blot, IHC, and ELISA all require…

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ELISA Protocol

ELISA Protocol

The Boster Bio protocol for ELISA offers a step-by-step overview of the procedure…

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ELISA Techniques

ELISA Protocol

There are five types of ELISA, thus, about ELISA protocol, a few differences exist….

ELISA Troubleshooting

Though without that complicated protocols as IHC, immunoprecipitation etc,…

ELISA Tips

This part is specially intended to provide some tips for ELISA…

Our Blog

30 Apr 2018

Guidelines for Preparing ELISA Standards

Keywords: enzyme-linked immunosorbent assay, protocols, troubleshooting,…

To set up a standard curve, ELISA standards should be carefully prepared for accurate sample… Read More

30 Apr 2018

5 Pitfalls to Avoid for ELISA!

Keywords: troubleshooting tips, technical, optimization

ELISA is a convenient and simple method to quantitatively or qualitatively detect peptides, proteins, antibodies,… Read More

30 Apr 2018

Which ELISA is for you?

Keywords: ELISA protocol, comparison table, technical…

Are you familiar with the multiple methods you could use to perform an ELISA? Among… Read More