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  • Grown antibodies are well validated with Human, Mouse and Rat tissues and in WB, IHC, ICC, Flow cytometry and ELISA. 
  • We also offer the following research products and services :

Products

Primary Antibodies

Secondary Antibodies

Picokine™ ELISA Kits

Multiplex Q-Plex™ ELISA Kits

Western Blotting Reagents

IHC Reagents

Recombinant Proteins

PCR and qPCR Products


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Services

Custom Antibodies (Rabbit/Mouse Polyclonal/Monoclonal)

Antibody Validation/Screening Service

Multiplex ELISA Testing Service

Histological Service

Gene Synthesis

Custom Recombinant Protein

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Affigen: Designing Antibodies and ELISA KitsProductsServicesCustom Rabbit Polyclonal Antibody ProductionCompany StatisticsCustom Monoclonal Antibody ServiceBoster HistoryProducts by Categories

Designing Antibodies and ELISA Kits


Always Improving

Gentaur has been offering high-quality antibodies and ELISA kits to the scientific community since 1993. Our antibodies are well-validated with Human, Mouse, and Rat tissues and used in various applications such as WB, IHC, ICC, Flow Cytometry, and ELISA.


Products

  •  Primary Antibodies
  • Secondary Antibodies
  • Picokine™ ELISA Kits
  • Multiplex Q-Plex™ ELISA Kits
  • Western Blotting Reagents
  • IHC Reagents
  • Recombinant Proteins
  • PCR and qPCR Products


Services

  •  Custom Antibodies (Rabbit/Mouse Polyclonal/Monoclonal)
  • Antibody Validation/Screening Service
  • Multiplex ELISA Testing Service
  • Histological Service
  • Gene Synthesis
  • Custom Recombinant Protein


Custom Rabbit Polyclonal Antibody Production

  • Price: $600
  • Antigen to Antibody for Non-Mammalian Models (Zebrafish, Drosophila, C. elegans, Yeast, etc.)
  • Production Time: 45 days


Company Statistics

  • Years of Service: 27
  • Publications: 23,000+
  • Rating: 4.8/5 (on biocompare.com)
  • Antibodies Produced: 16,000+
  • ELISA Kits Produced: 2,300+


Custom Monoclonal Antibody Service

  • Price: Starting at $4,000
  • Three-stage monoclonal antibody production service with continuous consultation to create an antibody suited for your specific application. Contact us for more details.


Boster History

  • With over 25 years of experience, Boster Bio was founded in 1993 by Steven Xia. We specialize in high-affinity, high-specificity antibodies and ELISA kits. Our products are cited in over 23,000 publications. Our antibodies undergo thorough validation for applications such as IHC, WB, ELISA, and Flow Cytometry. We are dedicated to providing exceptional service to researchers worldwide.


Products by Categories

  1. PRIMARY ANTIBODY
  2. PICOKINE™ ELISA KITS
  3. SECONDARY ANTIBODY KITS
  4. WESTERN BLOT REAGENTS
  5. IHC REAGENTS
  6. BEADS
  7. PROMOTIONS
  8. Buy Primary Get Secondary FREE
  9. ELISA Bulk Discount
  10. $20 For Your Review/Feedback
  11. TECHNICAL SUPPORT
  12. ELISA Principle
  13. Western Blot Principle
  14. IHC/ICC Principle
  15. ELISA Sample Preparation
  16. Western Blot sample Preparation
  17. IHC And ICC/IF Sample Preparation
  18. Western Blot Protocol
  19. IHC/ICC Protocols
  20. ChIP Protocol For Transcription Factors (TF)
  21. ChIP Protocol For Histones
  22. Picokine ™ ELISA Troubleshooting
  23. Western Blot Troubleshooting
  24. IHC/ICC Troubleshooting
  25. ChIP Troubleshooting Guide
  26. PRODUCTS BY RESEARCH AREAS
  27. Cytokine Network
  28. Alzheimer's Disease
  29. Diabetes
  30. Immunology
  31. Cancer
  32. Wnt Pathway
  33. AKT/PI3K/mTOR Pathway
  34. MAPK-p38 Signaling Pathway
  35. MAPK-JNK Signal Pathway


EasyStep ELISA

 

EasyStep Principle


The test principle applied in this kit is a Sandwich enzyme immunoassay. The microplate provided in this kit is pre-coated with a specific antibody. First, standard/samples are added to the plate, and proteins in the standards or samples bind to the antibodies on the microplate. Next, an HRP-conjugated antibody is added, followed by washing to remove unbound HRP-conjugated antibodies. Afterward, TMB substrate is added to initiate color development. The TMB is converted blue under the catalysis peroxidase action, turning yellow under acidic conditions. The color depth is positively related to the concentration of the target protein. Finally, a microplate reader is used to measure absorbance (OD value) at a wavelength of 450 nm, allowing the calculation of sample concentration by drawing a standard curve.

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Introduction to the Testing Methodology and Principle

This methodology, based on the test principle, involves the use of a microplate reader for measuring absorbance in order to determine the concentration of a target analyte in a sample. By leveraging the blue catalysis peroxidase reaction, this method offers a highly sensitive and efficient way to analyze biological samples. The TMB substrate, when converted to a blue color, plays a pivotal role in the assay’s ability to provide accurate results, offering a means to quantify the analyte concentration based on its reaction with peroxidase enzymes.



Catalysis Peroxidase and HRP-Conjugated Antibody Role

  • A key component of this method involves the use of HRP-conjugated antibody, which interacts with the analyte in the sample. When TMB is introduced, it undergoes a color development, transitioning to a blue catalysis peroxidase yellow color. 
  • This color change is directly related to the concentration of the analyte, and the microplate reader measures the absorbance OD value at a specific wavelength nm to determine the sample's concentration.

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Sandwich Enzyme Immunoassay Format

  • In this sandwich enzyme immunoassay, the test principle is applied through a streamlined, onestep sampling method that saves time and improves accuracy. The samples are processed using HRP-conjugated antibody, followed by the addition of the TMB substrate. 
  • This results in substrate color development, which is then measured to provide precise data on the concentration of the target analyte. The method’s efficiency lies in its ability to directly label the detection process, avoiding complex procedures and ensuring faster results.

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Three-Step Method for Sample Analysis

  • The three-step method enhances the test’s overall accuracy. It begins by adding standardsamples, followed by the addition of HRP-conjugated antibody. After washing to remove unbound antibodies, the final step involves adding TMB substrate to enable color development. 
  • The microplate reader measures the absorbance OD value at a defined wavelength nm, allowing for precise calculations of the sample's concentration.


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Efficiency, Advantages, and Conclusion


This method saves time and reduces operating errors, offering a more efficient process with limited samples, ensuring worry-free results. The advantages of using a three-step method include faster results, more reliable data, and a reduced risk of errors in sample analysis. The entire process is user-friendly, with clear guidance throughout to ensure accurate and reproducible results. For those interested in purchasing this method, Gentaur is an excellent option to obtain the necessary supplies and equipment.



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