ELISA Troubleshooting Low Sensitivity

Tip: For personalized troubleshooting assistance, contact us at support@bosterbio.com.

ELISA Troubleshooting: Low Sensitivity

When using small amounts of precious protein sample, the sensitivity of your ELISA assay can be its most important feature. Boster pre-coated ELISA kits are guaranteed to have consistently high sensitivity, but other ELISA formats can encounter errors that reduce their sensitivity. Use this troubleshooting guide to avoid common sources of reduced ELISA sensitivity:

ProblemCauseSolution
Incorrect assay typeAssay format inherently not sensitive enoughSwitch to a more sensitive detection system
Switch to a more sensitive assay type
Use Boster high-sensitivity ELISA kits
Incompatible sample typeUse sample types (e.g. serum vs. cell extract) verified to work as positive control
Include a positive control in your experiment
Reagent/sample problemsInsufficient targetReduce dilution factor or concentrate sample
Inactive substrateRun a control to verify reporter enzyme reacts with substrate
Insufficient substrateIncrease concentration of substrate
Mixing or substituting reagents from different kitsAvoid mixing components from different kits
Plate problemsImproper storage of ELISA kitFollow manufacturer storage recommendations
Poor target adsorption to wellsCovalently link target to wells

Keywords:- ELISA, troubleshooting, sensitivity, low sensitivity, low detection limit, sample not detected, no color development