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Western blotting, ELISAs, and IHC are all immunoassays, and so all share in common the same basic principle of operation: primary antibodies bind to the target protein of interest, then labelled secondary antibodies bind the primary antibody, allowing for the precise detection of protein.
ELISAs are much more precise and sensitive than other forms of immunoassay, capable of quantitatively detecting even the most minute concentrations of protein in a sample.
ELISA Sample Preparation
Every immunoassay protocol begins with sample preparation. Western blot, IHC, and ELISA all require different ways of preparing a sample, and nearly every sample type requires specialized treatment in order to produce high-quality, consistent results.
ELISAs can accurately assess soluble proteins in their native state, so are ideal for samples such as urine or saliva. Check out the ELISA sample preparation guides to learn how to get the best results from your sample type.
The Boster Bio protocol for ELISA offers a step-by-step overview of the procedure. Use this guide as a primer or a quick reference guide, and see our product datasheets or sample preparation guides for more detailed information.
ELISA Optimization Tips
Nearly every step of the ELISA procedure comes with a choice. Which sample preparation method is best? How concentrated should the antibodies be? Which blocking buffer will work best?
The Boster Bio optimization tips are meant to help answer those questions and more. Take a look at our optimization guides to help inform your decisions, optimize your experiments, and get better results.
ELISA Troubleshooting Tips
Every researcher eventually encounters trouble with an experiment. Blots appear blank, or IHC stains come out blurry, or ELISA standard curves come out wonky, or any of a thousand different problems.
Proper controls can help eliminate a great number of possible sources of error, but many troubles will remain. Check out the Boster troubleshooting guides for the most common troubles, their sources, and how to resolve them
ELISA Technical Blogs
Here is our ever growing archive of technical blog articles related to ELISA application. These include tips and tricks for experimental optimization, explanations of fundamental principles, more details on the why’s and how’s of ELISA protocols, related product recommendations, and more. Keep checking back for the most recent topics about ELISA research in the lab!