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30 Apr 2018

Guidelines for Preparing ELISA Standards

Keywords: enzyme-linked immunosorbent assay, protocols, troubleshooting, ELISA principles

To set up a standard curve, ELISA standards should be carefully prepared for accurate sample quantification of the target protein. We’ve provided some guidelines for you to consider when preparing ELISA standards. In this technical blog, you will learn what kind of standard you should choose, what the standard curve range should typically be, and how to properly prepare the standard dilutions. Read More

30 Apr 2018

5 Pitfalls to Avoid for ELISA!

Keywords: troubleshooting tips, technical, optimization

ELISA is a convenient and simple method to quantitatively or qualitatively detect peptides, proteins, antibodies, and hormones in samples, rendering it as one of the most widely used immunoassays. Despite the many advantages of conducting ELISA, there are some mistakes that could quickly turn your ELISA experiment sour. Read about the 5 common pitfalls to avoid when performing an ELISA so that you can prevent this situation from happening! Read More

30 Apr 2018

Which ELISA is for you?

Keywords: ELISA protocol, comparison table, technical tips, ELISA assay format, advantages of sandwich ELISA, indirect, direct, competitive immunoassay

Are you familiar with the multiple methods you could use to perform an ELISA? Among the standard assay formats, it is important to differentiate between the particular strategies that exist specifically for the detection step. An antigen could be captured to the plate by direct adsorption to the surface or through a pre-coated "capture" antibody, as in a sandwich ELISA. However, it is the detection step (direct or indirect detection) that largely determines the sensitivity of an ELISA. Read on to discover which ELISA is for you. Read More

30 Apr 2018

How to Use Checkerboard Titration to Optimize your ELISA Immunoassays

Keywords: ELISA assay optimization tips, checkboard titration principle explanation, ELISA protocol optimize, how to titrate

Optimizing your ELISA immunoassay can be a long and tedious process. Fortunately, using checkboard titration could help you optimize your ELISA assay more quickly, efficiently, and accurately. Here is a how-to-guide for utilizing this method in your next ELISA protocol. Read More

30 Apr 2018

ELISA Troubleshooting Saturated Signals

Keywords: ELISA immunoassay, technical tips, troubleshooting, ELISA excess signal, ELISA kit result optimization

Every ELISA kit user comes across the need to troubleshoot their assay at some point. Saturated signals can result from many different possible causes, and this could make troubleshooting complicated. Dr. Booster has come up with a table of recommended solutions for troubleshooting your ELISA assay. If this table does not help your specific experiment, please feel free to contact Boster's technical support at any time. Get better results with Boster! Read More